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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated GEN1 Monoclonal antibody

GEN1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A10D10

Cat no : CL488-68060

Synonyms

GEN1, 1A10D10, Gen

Validation Data Gallery

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  • Flow cytometry (FC) experiment of A431 cells using CoraLite® Plus 488-conjugated GEN1 Monoclonal anti (CL488-68060)

    FC experiment of A431 using CL488-68060

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human GEN1 (CL488-68060, Clone:1A10D10) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive FC (Intra) detected inA431 cells
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68060 targets GEN1 in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GEN1 fusion protein Ag31247 相同性解析による交差性が予測される生物種
Full Name Gen homolog 1, endonuclease (Drosophila)
Calculated molecular weight 103 kDa
Observed molecular weight98-100 kDa
GenBank accession numberNM_182625
Gene symbol GEN1
Gene ID (NCBI) 348654
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GEN1 is a endonuclease which resolves Holliday junctions (HJs) by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. GEN1 efficiently cleaves both single and double HJs contained within large recombination intermediates.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 GEN1 antibody CL488-68060Download protocol
Standard Protocols
Click here to view our Standard Protocols