CoraLite® Plus 647-conjugated GFP tag Monoclonal antibody
GFP tag Monoclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Mouse / IgG2a
Reactivity
recombinant protein
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
1E10H7
Cat no : CL647-66002
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | Transfected HEK-293 cells |
| Positive FC (Intra) detected in | Transfected HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL647-66002 targets GFP tag in IF/ICC, FC (Intra) applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | GFP tag fusion protein Ag2128 相同性解析による交差性が予測される生物種 |
| Full Name | GFP tag |
| Calculated molecular weight | 26 kDa |
| GenBank accession number | M62653 |
| Gene symbol | |
| Gene ID (NCBI) | |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages.
Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm.
When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.
This antibody is a mouse (IgG2a) monoclonal antibody against GFP reactive to GFP, eGFP, eYFP, YFP and CFP. This antibody is labeled with CoraLite Plus 647.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 647 GFP tag antibody CL647-66002 | Download protocol |
| FC protocol for CL Plus 647 GFP tag antibody CL647-66002 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
