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  • Featured Product
  • KD/KO Validated

GGA2 Polyclonal antibody, PBS Only

GGA2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10356-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using GGA2 Polyclonal antibody (10356-1-AP)

    WB analysis of HeLa using 10356-1-AP (same clone as 10356-1-PBS)

    WB result of GGA2 antibody (10356-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GGA2 transfected HeLa cells. This data was developed using the same antibody clone with 10356-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using GGA2 Polyclonal antibody (10356-1-AP)

    WB analysis of mouse brain using 10356-1-AP (same clone as 10356-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10356-1-AP (GGA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10356-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using GGA2 Polyclonal antibody (10356-1-AP)

    WB analysis of human kidney using 10356-1-AP (same clone as 10356-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 10356-1-AP (GGA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10356-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse adipose tissue using GGA2 Polyclonal antibody (10356-1-AP)

    WB analysis of mouse adipose using 10356-1-AP (same clone as 10356-1-PBS)

    mouse adipose tissue were subjected to SDS PAGE followed by western blot with 10356-1-AP (GGA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10356-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using GGA2 Polyclonal antibody (10356-1-AP)

    IHC staining of human colon cancer using 10356-1-AP (same clone as 10356-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10356-1-AP (GGA2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10356-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using GGA2 Polyclonal antibody (10356-1-AP)

    IHC staining of human stomach cancer using 10356-1-AP (same clone as 10356-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10356-1-AP (GGA2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10356-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GGA2 Polyclonal antibody (10356-1-AP)

    IF Staining of HeLa using 10356-1-AP (same clone as 10356-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GGA2 antibody (10356-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10356-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10356-1-PBS targets GGA2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GGA2 fusion protein Ag0370 相同性解析による交差性が予測される生物種
Full Name golgi associated, gamma adaptin ear containing, ARF binding protein 2
Calculated molecular weight 67 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC000284
Gene symbol GGA2
Gene ID (NCBI) 23062
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GGA2, also known as VEAR, belongs to the GGA protein family and regulates the trafficking of proteins between the trans-Golgi network and the lysosome. GGA2 has an amino-terminal VHS domain in its N terminus which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins.