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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GHITM Recombinant antibody (83548-3-RR)

    WB analysis using 83548-3-RR (same clone as 83548-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83548-3-RR (GHITM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83548-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GHITM Recombinant antibody (83548-3-RR)

    WB analysis using 83548-3-RR (same clone as 83548-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83548-3-RR (GHITM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83548-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using GHITM Recombinant antibody (83548-3-RR)

    FC experiment of HeLa using 83548-3-RR (same clone as 83548-3-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug GHITM Recombinant antibody (83548-3-RR, Clone:240541D3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83548-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00524-1

    Cytometric bead array standard curve of MP00524-1

    Cytometric bead array standard curve of MP00524-1, GHITM Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83548-2-PBS. Detection antibody: 83548-3-PBS. Standard: Ag35170. Range: 0.78-100 ng/mL.

  • Cytometric bead array standard curve of MP00524-2

    Cytometric bead array standard curve of MP00524-2

    Cytometric bead array standard curve of MP00524-2, GHITM Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83548-1-PBS. Detection antibody: 83548-3-PBS. Standard: Ag35170. Range: 0.78-100 ng/mL.

  • Affinity and Kinetic Characterization of 83548-3-RR

    Affinity and Kinetic Characterization of 83548-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83548-3-RR against Human GHITM were performed. The affinity constant is 2.41 nM.

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GHITM Recombinant antibody, PBS Only (Detector)

GHITM Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83548-3-PBS
Clone No.240541D3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

My021, MICS1, Growth hormone-inducible transmembrane protein, DERP2, Dermal papilla-derived protein 2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83548-3-PBS targets GHITM as part of a matched antibody pair:

MP00524-1: 83548-2-PBS capture and 83548-3-PBS detection (validated in Cytometric bead array)

MP00524-2: 83548-1-PBS capture and 83548-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GHITM fusion protein Ag35170 相同性解析による交差性が予測される生物種
Full Name growth hormone inducible transmembrane protein
Calculated molecular weight 345 aa, 37 kDa
Observed molecular weight37 kDa
GenBank accession numberBC010354
Gene Symbol GHITM
Gene ID (NCBI) 27069
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H3K2
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

GHITM, also known as MICS1, TMBIM5 or DERP2, is a mitochondrial protein that localizes in the inner membrane. GHITM is involved in mitochondrial morphology in specific cristae structures and the apoptotic release of cytochrome c from the mitochondria (PMID: 18417609). The gene of GHITM maps to chromosome 10q23.1, and encodes a 345-amino-acid protein with a calculated molecular mass of 37 kDa.