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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GHITM Recombinant antibody (83548-3-RR)

    WB analysis using 83548-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83548-3-RR (GHITM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using GHITM Recombinant antibody (83548-3-RR)

    WB analysis using 83548-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83548-3-RR (GHITM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using GHITM Recombinant antibody (83548-3-RR)

    FC experiment of HeLa using 83548-3-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug GHITM Recombinant antibody (83548-3-RR, Clone:240541D3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83548-3-RR

    Affinity and Kinetic Characterization of 83548-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83548-3-RR against Human GHITM were performed. The affinity constant is 2.41 nM.

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GHITM Recombinant antibody

GHITM Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83548-3-RR
Clone No.240541D3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Synonyms

My021, MICS1, Growth hormone-inducible transmembrane protein, DERP2, Dermal papilla-derived protein 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293T cells, SH-SY5Y cells, mouse liver tissue
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:600-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83548-3-RR targets GHITM in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GHITM fusion protein Ag35170 相同性解析による交差性が予測される生物種
Full Name growth hormone inducible transmembrane protein
Calculated molecular weight 345 aa, 37 kDa
Observed molecular weight37 kDa
GenBank accession numberBC010354
Gene Symbol GHITM
Gene ID (NCBI) 27069
RRIDAB_3671167
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H3K2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GHITM, also known as MICS1, TMBIM5 or DERP2, is a mitochondrial protein that localizes in the inner membrane. GHITM is involved in mitochondrial morphology in specific cristae structures and the apoptotic release of cytochrome c from the mitochondria (PMID: 18417609). The gene of GHITM maps to chromosome 10q23.1, and encodes a 345-amino-acid protein with a calculated molecular mass of 37 kDa.

Protocols

Product Specific Protocols
WB protocol for GHITM antibody 83548-3-RRDownload protocol
FC protocol for GHITM antibody 83548-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols