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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using GIMAP4 Polyclonal antibody (11852-1-AP)

    WB analysis of Jurkat using 11852-1-AP (same clone as 11852-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11852-1-AP (GIMAP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11852-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Jurkat cells using GIMAP4 Polyclonal antibody (11852-1-AP)

    IP experiment of Jurkat using 11852-1-AP (same clone as 11852-1-PBS)

    IP result of anti-GIMAP4 (IP:11852-1-AP, 3ug; Detection:11852-1-AP 1:800) with Jurkat cells lysate 1080ug. This data was developed using the same antibody clone with 11852-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using GIMAP4 Polyclonal antibody (11852-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 11852-1-AP (same clone as 11852-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 11852-1-AP (GIMAP4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11852-1-PBS in a different storage buffer formulation.

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GIMAP4 Polyclonal antibody, PBS Only

GIMAP4 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 11852-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11852-1-PBS targets GIMAP4 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GIMAP4 fusion protein Ag2422 相同性解析による交差性が予測される生物種
Full Name GTPase, IMAP family member 4
Calculated molecular weight 329 aa, 38 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC020657
Gene Symbol GIMAP4
Gene ID (NCBI) 55303
RRIDAB_2279105
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NUV9
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

GIMAP4, also named as hIAN1 or HIMAP4, is a member of GIMAP family. Among GIMAP family, GIMAP4 is the only member reported to have true GTPase activity, and its transcription is found to be differentially regulated during early human CD4+ T helper (Th) lymphocyte differentiation. GIMAP4 has been previously connected mainly with T- and B-cell development and survival and T-cell apoptosis. The MW of this protein is 38 kDa, and Catalog#11852-1-AP specially recognises the 38 kDa protein.