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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GLO1 Monoclonal antibody (68242-1-Ig)

    WB analysis using 68242-1-Ig (same clone as 68242-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68242-1-Ig (GLO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68242-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of LNCaP cells using GLO1 Monoclonal antibody (68242-1-Ig)

    WB analysis of LNCaP using 68242-1-Ig (same clone as 68242-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68242-1-Ig (GLO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68242-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GLO1 Monoclonal antibody (68242-1-Ig)

    WB analysis using 68242-1-Ig (same clone as 68242-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68242-1-Ig (GLO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68242-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GLO1 Monoclonal antibody (68242-1-Ig)

    IF Staining of HeLa using 68242-1-Ig (same clone as 68242-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GLO1 antibody (68242-1-Ig, Clone: 1B7A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68242-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using GLO1 Monoclonal antibody (68242-1-Ig)

    FC experiment of HeLa using 68242-1-Ig (same clone as 68242-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug GLO1 Monoclonal antibody (68242-1-Ig, Clone:1B7A8) and CoraLite488-conjugated Donkey Anti-Mouse IgG(H+L)( SA00013-5)(red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68242-1-PBS in a different storage buffer formulation.

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GLO1 Monoclonal antibody, PBS Only

GLO1 Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 68242-1-PBS
Clone No.1B7A8

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

GLYI, Glx I, GLOD1, ELNR1, EC:4.4.1.5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1mg/ml
100 μg, 1mg/ml

¥167,000/ 100 μg

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68242-1-PBS targets GLO1 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen GLO1 fusion protein Ag7314 相同性解析による交差性が予測される生物種
Full Name glyoxalase I
Calculated molecular weight 21 kDa
Observed molecular weight 21-25 kDa
GenBank accession numberBC001741
Gene Symbol GLO1
Gene ID (NCBI) 2739
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ04760
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Glo1 (glyxoalase I) is a cytosolic protein expressed in all mammalian cells. Its physiological function is the detoxification of MG (methylglyoxal), which is a potent precursor of AGEs (advanced glycation end-products). Glo1 is known to play a role in many diseases including diabetes mellitus, Alzheimer's disease, and cancer.