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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GLO1 Monoclonal antibody

GLO1 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1B7A8

Cat no : 68242-1-Ig

Synonyms

GLYI, Glx I, GLOD1, ELNR1, EC:4.4.1.5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GLO1 Monoclonal antibody (68242-1-Ig)

    WB analysis using 68242-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68242-1-Ig (GLO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of LNCaP cells using GLO1 Monoclonal antibody (68242-1-Ig)

    WB analysis of LNCaP using 68242-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68242-1-Ig (GLO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using GLO1 Monoclonal antibody (68242-1-Ig)

    WB analysis using 68242-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68242-1-Ig (GLO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GLO1 Monoclonal antibody (68242-1-Ig)

    IF Staining of HeLa using 68242-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GLO1 antibody (68242-1-Ig, Clone: 1B7A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using GLO1 Monoclonal antibody (68242-1-Ig)

    FC experiment of HeLa using 68242-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug GLO1 Monoclonal antibody (68242-1-Ig, Clone:1B7A8) and CoraLite488-conjugated Donkey Anti-Mouse IgG(H+L)( SA00013-5)(red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHEK-293 cells, LNCaP cells, Jurkat cells, HepG2 cells, K-562 cells, HSC-T6 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68242-1-Ig targets GLO1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen GLO1 fusion protein Ag7314 相同性解析による交差性が予測される生物種
Full Name glyoxalase I
Calculated molecular weight 21 kDa
Observed molecular weight 21-25 kDa
GenBank accession numberBC001741
Gene symbol GLO1
Gene ID (NCBI) 2739
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glo1 (glyxoalase I) is a cytosolic protein expressed in all mammalian cells. Its physiological function is the detoxification of MG (methylglyoxal), which is a potent precursor of AGEs (advanced glycation end-products). Glo1 is known to play a role in many diseases including diabetes mellitus, Alzheimer's disease, and cancer.

Protocols

Product Specific Protocols
WB protocol for GLO1 antibody 68242-1-IgDownload protocol
IF protocol for GLO1 antibody 68242-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols