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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated GLO1 Monoclonal antibody

GLO1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B7A8

Cat no : CL488-68242

Synonyms

GLYI, Glx I, GLOD1, ELNR1, EC:4.4.1.5

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated GLO1 Monoclonal anti (CL488-68242)

    IF Staining of HeLa using CL488-68242

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 GLO1 antibody (CL488-68242, Clone: 1B7A8 ) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated GLO1 Monoclonal anti (CL488-68242)

    FC experiment of HeLa using CL488-68242

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Glo1 Monoclonal Antibody (CL488-68242, Clone:1B7A8)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68242 targets GLO1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen GLO1 fusion protein Ag7314 相同性解析による交差性が予測される生物種
Full Name glyoxalase I
Calculated molecular weight 21 kDa
Observed molecular weight 21-25 kDa
GenBank accession numberBC001741
Gene symbol GLO1
Gene ID (NCBI) 2739
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glo1 (glyxoalase I) is a cytosolic protein expressed in all mammalian cells. Its physiological function is the detoxification of MG (methylglyoxal), which is a potent precursor of AGEs (advanced glycation end-products). Glo1 is known to play a role in many diseases including diabetes mellitus, Alzheimer's disease, and cancer.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 GLO1 antibody CL488-68242Download protocol
FC protocol for CL Plus 488 GLO1 antibody CL488-68242Download protocol
Standard Protocols
Click here to view our Standard Protocols