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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using GM2A Monoclonal antibody (66080-1-Ig)

    WB analysis of HeLa using 66080-1-Ig (same clone as 66080-1-PBS)

    WB result of GM2A antibody (66080-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GM2A transfected HeLa cells. This data was developed using the same antibody clone with 66080-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using GM2A Monoclonal antibody (66080-1-Ig)

    WB analysis of HeLa using 66080-1-Ig (same clone as 66080-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66080-1-Ig (GM2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66080-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using GM2A Monoclonal antibody (66080-1-Ig)

    WB analysis of human placenta using 66080-1-Ig (same clone as 66080-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66080-1-Ig (GM2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66080-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293 cells using GM2A Monoclonal antibody (66080-1-Ig)

    FC experiment of HEK-293 using 66080-1-Ig (same clone as 66080-1-PBS)

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human GM2A (66080-1-Ig, Clone:1E4D3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66080-1-PBS in a different storage buffer formulation.

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  • KD/KO Validated

GM2A Monoclonal antibody, PBS Only

GM2A Monoclonal Antibody for ELISA, WB
Cat No. 66080-1-PBS
Clone No.1E4D3

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

ELISA, WB

Synonyms

SAP-3, SAP3, GM2-AP, GM2AP, Ganglioside GM2 activator isoform short

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66080-1-PBS targets GM2A in ELISA, WB applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen GM2A fusion protein Ag4394 相同性解析による交差性が予測される生物種
Full Name GM2 ganglioside activator
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC009273
Gene Symbol GM2A
Gene ID (NCBI) 2760
RRIDAB_11182593
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP17900
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

GM2A, also named as GM2-AP and SAP-3, is the large binding pocket which can accommodate several single chain phospholipids and fatty acids. GM2A also exhibits some calcium-independent phospholipase activity. It plays a key role in the degradation of ganglioside GM2 (GM2). GM2A stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A.