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GM2A Monoclonal antibody

GM2A Monoclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1E4D3

Cat no : 66080-1-Ig

Synonyms

SAP-3, SAP3, GM2-AP, GM2AP, Ganglioside GM2 activator isoform short

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using GM2A Monoclonal antibody (66080-1-Ig)

    WB analysis of HeLa using 66080-1-Ig

    WB result of GM2A antibody (66080-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GM2A transfected HeLa cells.

  • Western Blot (WB) analysis of HeLa cells using GM2A Monoclonal antibody (66080-1-Ig)

    WB analysis of HeLa using 66080-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66080-1-Ig (GM2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using GM2A Monoclonal antibody (66080-1-Ig)

    WB analysis of human placenta using 66080-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66080-1-Ig (GM2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HEK-293 cells using GM2A Monoclonal antibody (66080-1-Ig)

    FC experiment of HEK-293 using 66080-1-Ig

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human GM2A (66080-1-Ig, Clone:1E4D3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHela cells, human placenta tissue
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66080-1-Ig targets GM2A in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen GM2A fusion protein Ag4394 相同性解析による交差性が予測される生物種
Full Name GM2 ganglioside activator
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC009273
Gene symbol GM2A
Gene ID (NCBI) 2760
RRIDAB_11182593
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GM2A, also named as GM2-AP and SAP-3, is the large binding pocket which can accommodate several single chain phospholipids and fatty acids. GM2A also exhibits some calcium-independent phospholipase activity. It plays a key role in the degradation of ganglioside GM2 (GM2). GM2A stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A.

Protocols

Product Specific Protocols
WB protocol for GM2A antibody 66080-1-IgDownload protocol
FC protocol for GM2A antibody 66080-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols