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Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated GM2A Monoclonal anti (CL488-66080)

    FC experiment of HeLa using CL488-66080

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Gm2a Monoclonal Antibody (CL488-66080, Clone:1E4D3)(red), or 0.4 ug Isotype Control (CL488-66360-2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  • KD/KO Validated

CoraLite® Plus 488-conjugated GM2A Monoclonal antibody

GM2A Monoclonal Antibody for FC (Intra)
Cat No. CL488-66080
Clone No.1E4D3

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC (Intra)

Synonyms

SAP-3, SAP3, GM2-AP, GM2AP, Ganglioside GM2 activator isoform short

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66080 targets GM2A in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen GM2A fusion protein Ag4394 相同性解析による交差性が予測される生物種
Full Name GM2 ganglioside activator
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC009273
Gene Symbol GM2A
Gene ID (NCBI) 2760
RRIDAB_3672903
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP17900
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GM2A, also named as GM2-AP and SAP-3, is the large binding pocket which can accommodate several single chain phospholipids and fatty acids. GM2A also exhibits some calcium-independent phospholipase activity. It plays a key role in the degradation of ganglioside GM2 (GM2). GM2A stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 GM2A antibody CL488-66080Download protocol
Standard Protocols
Click here to view our Standard Protocols