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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GMNN Monoclonal antibody, PBS Only

GMNN Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E3A2

Cat no : 66566-1-PBS

Synonyms

Gem, Geminin, GMNN, RP3 369A17.3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using GMNN Monoclonal antibody (66566-1-Ig)

    WB analysis using 66566-1-Ig (same clone as 66566-1-PBS)

    Various cells were subjected to SDS PAGE followed by western blot with 66566-1-Ig (GMNN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66566-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GMNN Monoclonal antibody (66566-1-Ig)

    WB analysis using 66566-1-Ig (same clone as 66566-1-PBS)

    Various cells were subjected to SDS PAGE followed by western blot with 66566-1-Ig (GMNN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66566-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using GMNN Monoclonal antibody (66566-1-Ig)

    IHC staining of human colon cancer using 66566-1-Ig (same clone as 66566-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66566-1-Ig (GMNN antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66566-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using GMNN Monoclonal antibody (66566-1-Ig)

    IHC staining of human tonsillitis using 66566-1-Ig (same clone as 66566-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66566-1-Ig (GMNN antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66566-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using GMNN Monoclonal antibody (66566-1-Ig)

    IHC staining of human tonsillitis using 66566-1-Ig (same clone as 66566-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66566-1-Ig (GMNN antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66566-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using GMNN Monoclonal antibody (66566-1-Ig)

    IHC staining of human colon cancer using 66566-1-Ig (same clone as 66566-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66566-1-Ig (GMNN antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66566-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66566-1-PBS targets GMNN in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GMNN fusion protein Ag24283 相同性解析による交差性が予測される生物種
Full Name geminin, DNA replication inhibitor
Calculated molecular weight 24 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC005185
Gene symbol GMNN
Gene ID (NCBI) 51053
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GMNN, also designated geminin, contains 212 amino acids and has a destruction box sequence (RRTLKVIQP). GMNN participates in ininhibiting DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the metaphase-anaphase transition of cell cycle's mitotic phase, which permits replication in the succeeding cell cycle. GMNN has a broad sedimentation profile ranging from about 25 kDa to 90 kDa, with a major peak at 30 kDa. Scanning of the signals shows that discrete peaks corresponding to apparent mass of 42.5 kDa and 66 kDa are present. (PMID: 15313623)