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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GMNN Monoclonal antibody

GMNN Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2E3A2

Cat no : 66566-1-Ig

Synonyms

Gem, Geminin, GMNN, RP3 369A17.3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GMNN Monoclonal antibody (66566-1-Ig)

    WB analysis using 66566-1-Ig

    Various cells were subjected to SDS PAGE followed by western blot with 66566-1-Ig (GMNN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using GMNN Monoclonal antibody (66566-1-Ig)

    WB analysis using 66566-1-Ig

    Various cells were subjected to SDS PAGE followed by western blot with 66566-1-Ig (GMNN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using GMNN Monoclonal antibody (66566-1-Ig)

    IHC staining of human colon cancer using 66566-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66566-1-Ig (GMNN antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using GMNN Monoclonal antibody (66566-1-Ig)

    IHC staining of human tonsillitis using 66566-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66566-1-Ig (GMNN antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using GMNN Monoclonal antibody (66566-1-Ig)

    IHC staining of human tonsillitis using 66566-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66566-1-Ig (GMNN antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using GMNN Monoclonal antibody (66566-1-Ig)

    IHC staining of human colon cancer using 66566-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66566-1-Ig (GMNN antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, L02 cells, HEK-293 cells, NCCIT cells, Jurkat cells
Positive IHC detected inhuman colon cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66566-1-Ig targets GMNN in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GMNN fusion protein Ag24283 相同性解析による交差性が予測される生物種
Full Name geminin, DNA replication inhibitor
Calculated molecular weight 24 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC005185
Gene symbol GMNN
Gene ID (NCBI) 51053
RRIDAB_2881927
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GMNN, also designated geminin, contains 212 amino acids and has a destruction box sequence (RRTLKVIQP). GMNN participates in ininhibiting DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the metaphase-anaphase transition of cell cycle's mitotic phase, which permits replication in the succeeding cell cycle. GMNN has a broad sedimentation profile ranging from about 25 kDa to 90 kDa, with a major peak at 30 kDa. Scanning of the signals shows that discrete peaks corresponding to apparent mass of 42.5 kDa and 66 kDa are present. (PMID: 15313623)

Protocols

Product Specific Protocols
WB protocol for GMNN antibody 66566-1-IgDownload protocol
IHC protocol for GMNN antibody 66566-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Cell Rep

Increased degradation of FMRP contributes to neuronal hyperexcitability in tuberous sclerosis complex

Authors - Kellen D Winden
View Article