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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated GMNN Monoclonal antibody (CL594-66566)

    IF Staining of HeLa using CL594-66566

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 GMNN antibody (CL594-66566, Clone: 2E3A2 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated GMNN Monoclonal antibody (CL594-66566)

    IF Staining of HepG2 using CL594-66566

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 GMNN antibody (CL594-66566, Clone: 2E3A2 ) at dilution of 1:200.

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CoraLite®594-conjugated Geminin Monoclonal antibody

Geminin Monoclonal Antibody for IF/ICC
Cat No. CL594-66566
Clone No.2E3A2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Synonyms

GMNN, MGORS6, Gem, 2E3A2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66566 targets Geminin in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Geminin fusion protein Ag24283 相同性解析による交差性が予測される生物種
Full Name geminin, DNA replication inhibitor
Calculated molecular weight 24 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC005185
Gene Symbol GMNN
Gene ID (NCBI) 51053
RRIDAB_2920007
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO75496
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GMNN, also designated geminin, contains 212 amino acids and has a destruction box sequence (RRTLKVIQP). GMNN participates in ininhibiting DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the metaphase-anaphase transition of cell cycle's mitotic phase, which permits replication in the succeeding cell cycle. GMNN has a broad sedimentation profile ranging from about 25 kDa to 90 kDa, with a major peak at 30 kDa. Scanning of the signals shows that discrete peaks corresponding to apparent mass of 42.5 kDa and 66 kDa are present. (PMID: 15313623)

Protocols

Product Specific Protocols
IF protocol for CL594 Geminin antibody CL594-66566Download protocol
Standard Protocols
Click here to view our Standard Protocols