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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GNAQ Monoclonal antibody (68191-1-Ig)

    WB analysis using 68191-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68191-1-Ig (GNAQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig cerebellum tissue using GNAQ Monoclonal antibody (68191-1-Ig)

    WB analysis of pig cerebellum using 68191-1-Ig

    pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 68191-1-Ig (GNAQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using GNAQ Monoclonal antibody (68191-1-Ig)

    WB analysis using 68191-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68191-1-Ig (GNAQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat cerebellum tissue using GNAQ Monoclonal antibody (68191-1-Ig)

    WB analysis of rat cerebellum using 68191-1-Ig

    rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 68191-1-Ig (GNAQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GNAQ Monoclonal antibody (68191-1-Ig)

    WB analysis of HeLa using 68191-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 68191-1-Ig (GNAQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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GNAQ Monoclonal antibody

GNAQ Monoclonal Antibody for WB, ELISA
Cat No. 68191-1-Ig
Clone No.2H5G6

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Rabbit, Pig

Applications

WB, ELISA

Synonyms

G ALPHA q, GAQ, GNAQ

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, pig cerebellum tissue, rat brain tissue, rat cerebellum tissue, HeLa cells, pig brain tissue, rabbit brain tissue, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68191-1-Ig targets GNAQ in WB, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

Tested Reactivity Human, Mouse, Rat, Rabbit, Pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen GNAQ fusion protein Ag26178 相同性解析による交差性が予測される生物種
Full Name guanine nucleotide binding protein (G protein), q polypeptide
Calculated molecular weight 42 kDa
Observed molecular weight 38-40 kDa
GenBank accession numberBC057777
Gene Symbol GNAQ
Gene ID (NCBI) 2776
RRIDAB_2935280
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP50148
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GNAQ is a proto-oncogene closely related to the α subunit of guanine nucleotide-binding proteins (G proteins) that serve as key intermediates between membrane-bound G-protein-coupled receptors (GPCRs) and GPCR signalling nodes. Aberrant expression and activity of G proteins and GPCRs are frequently associated with tumorigenesis. Recent studies show that oncogenic activating mutations in GNAQ are present in 80% of the melanomas. The calculated molecular weight of GNAQ is 42 kDa. This antibody can detect a band of 38-40 kDa in SDS-PAGE. (PMID: 33993733)

Protocols

Product Specific Protocols
WB protocol for GNAQ antibody 68191-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols