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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GNAQ Monoclonal antibody, PBS Only

GNAQ Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Rabbit, Pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2H5G6

Cat no : 68191-1-PBS

Synonyms

G ALPHA q, GAQ, GNAQ

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using GNAQ Monoclonal antibody (68191-1-Ig)

    WB analysis using 68191-1-Ig (same clone as 68191-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68191-1-Ig (GNAQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68191-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig cerebellum tissue using GNAQ Monoclonal antibody (68191-1-Ig)

    WB analysis of pig cerebellum using 68191-1-Ig (same clone as 68191-1-PBS)

    pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 68191-1-Ig (GNAQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68191-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GNAQ Monoclonal antibody (68191-1-Ig)

    WB analysis using 68191-1-Ig (same clone as 68191-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68191-1-Ig (GNAQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68191-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat cerebellum tissue using GNAQ Monoclonal antibody (68191-1-Ig)

    WB analysis of rat cerebellum using 68191-1-Ig (same clone as 68191-1-PBS)

    rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 68191-1-Ig (GNAQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68191-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using GNAQ Monoclonal antibody (68191-1-Ig)

    WB analysis of HeLa using 68191-1-Ig (same clone as 68191-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 68191-1-Ig (GNAQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68191-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68191-1-PBS targets GNAQ in WB, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

Tested Reactivity Human, Mouse, Rat, Rabbit, Pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen GNAQ fusion protein Ag26178 相同性解析による交差性が予測される生物種
Full Name guanine nucleotide binding protein (G protein), q polypeptide
Calculated molecular weight 42 kDa
Observed molecular weight 38-40 kDa
GenBank accession numberBC057777
Gene symbol GNAQ
Gene ID (NCBI) 2776
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

GNAQ is a proto-oncogene closely related to the α subunit of guanine nucleotide-binding proteins (G proteins) that serve as key intermediates between membrane-bound G-protein-coupled receptors (GPCRs) and GPCR signalling nodes. Aberrant expression and activity of G proteins and GPCRs are frequently associated with tumorigenesis. Recent studies show that oncogenic activating mutations in GNAQ are present in 80% of the melanomas. The calculated molecular weight of GNAQ is 42 kDa. This antibody can detect a band of 38-40 kDa in SDS-PAGE. (PMID: 33993733)