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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GNB3 Monoclonal antibody

GNB3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2B6E1

Cat no : 67497-1-Ig

Synonyms

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3, 2B6E1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GNB3 Monoclonal antibody (67497-1-Ig)

    WB analysis using 67497-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67497-1-Ig (GNB3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using GNB3 Monoclonal antibody (67497-1-Ig)

    WB analysis using 67497-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67497-1-Ig (GNB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using GNB3 Monoclonal antibody (67497-1-Ig)

    WB analysis of L02 using 67497-1-Ig

    L02 cells were subjected to SDS PAGE followed by western blot with 67497-1-Ig (GNB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using GNB3 Monoclonal antibody (67497-1-Ig)

    WB analysis of HepG2 using 67497-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67497-1-Ig (GNB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat retina tissue using GNB3 Monoclonal antibody (67497-1-Ig)

    WB analysis of rat retina using 67497-1-Ig

    rat retina tissue were subjected to SDS PAGE followed by western blot with 67497-1-Ig (GNB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GNB3 Monoclonal antibody (67497-1-Ig)

    IHC staining of human liver cancer using 67497-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67497-1-Ig (GNB3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GNB3 Monoclonal antibody (67497-1-Ig)

    IHC staining of human liver cancer using 67497-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67497-1-Ig (GNB3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GNB3 Monoclonal antibody (67497-1-Ig)

    IF Staining of HepG2 using 67497-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GNB3 antibody (67497-1-Ig, Clone: 2B6E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inhTERT-RPE1 cells, HepG2 cells, L02 cells, pig brain tissue, rat retina tissue, Raji cells, Ramos cells, Daudi cells, RKO cells, Karpas-422 cells, IMR-32 cells, Rat brain cells, Mouse brain cells, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67497-1-Ig targets GNB3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GNB3 fusion protein Ag7050 相同性解析による交差性が予測される生物種
Full Name guanine nucleotide binding protein (G protein), beta polypeptide 3
Calculated molecular weight 37 kDa
Observed molecular weight 35-37 kDa
GenBank accession numberBC002454
Gene symbol GNB3
Gene ID (NCBI) 2784
RRIDAB_2882721
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Guanine nucleotide-binding proteins (g proteins) are involved as a modulator or transducer in various transmembrane signaling systems, by integrating signals between receptors and effector proteins. G proteins are composed of an alpha, a beta, and a gamma subunit. This gene encodes a 34 kd beta subunit, being expressed in all tissues. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors.

Protocols

Product Specific Protocols
WB protocol for GNB3 antibody 67497-1-IgDownload protocol
IHC protocol for GNB3 antibody 67497-1-IgDownload protocol
IF protocol for GNB3 antibody 67497-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols