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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GNB3 Monoclonal antibody (67497-1-Ig)

    WB analysis using 67497-1-Ig (same clone as 67497-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67497-1-Ig (GNB3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67497-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GNB3 Monoclonal antibody (67497-1-Ig)

    WB analysis using 67497-1-Ig (same clone as 67497-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67497-1-Ig (GNB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67497-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using GNB3 Monoclonal antibody (67497-1-Ig)

    WB analysis of L02 using 67497-1-Ig (same clone as 67497-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 67497-1-Ig (GNB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67497-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using GNB3 Monoclonal antibody (67497-1-Ig)

    WB analysis of HepG2 using 67497-1-Ig (same clone as 67497-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67497-1-Ig (GNB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67497-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat retina tissue using GNB3 Monoclonal antibody (67497-1-Ig)

    WB analysis of rat retina using 67497-1-Ig (same clone as 67497-1-PBS)

    rat retina tissue were subjected to SDS PAGE followed by western blot with 67497-1-Ig (GNB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67497-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GNB3 Monoclonal antibody (67497-1-Ig)

    IHC staining of human liver cancer using 67497-1-Ig (same clone as 67497-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67497-1-Ig (GNB3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67497-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GNB3 Monoclonal antibody (67497-1-Ig)

    IHC staining of human liver cancer using 67497-1-Ig (same clone as 67497-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67497-1-Ig (GNB3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67497-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GNB3 Monoclonal antibody (67497-1-Ig)

    IF Staining of HepG2 using 67497-1-Ig (same clone as 67497-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GNB3 antibody (67497-1-Ig, Clone: 2B6E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67497-1-PBS in a different storage buffer formulation.

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GNB3 Monoclonal antibody, PBS Only

GNB3 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67497-1-PBS
Clone No.2B6E1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3, 2B6E1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67497-1-PBS targets GNB3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GNB3 fusion protein Ag7050 相同性解析による交差性が予測される生物種
Full Name guanine nucleotide binding protein (G protein), beta polypeptide 3
Calculated molecular weight 37 kDa
Observed molecular weight 35-37 kDa
GenBank accession numberBC002454
Gene Symbol GNB3
Gene ID (NCBI) 2784
RRIDAB_2882721
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP16520
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Guanine nucleotide-binding proteins (g proteins) are involved as a modulator or transducer in various transmembrane signaling systems, by integrating signals between receptors and effector proteins. G proteins are composed of an alpha, a beta, and a gamma subunit. This gene encodes a 34 kd beta subunit, being expressed in all tissues. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors.