GNE Monoclonal antibody
GNE Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, rat and More (1)
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
2A11E10
Cat no : 67649-1-Ig
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | A549 cells, Hela cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
67649-1-Ig targets GNE in WB, ELISA applications and shows reactivity with Human, rat samples.
| Tested Reactivity | Human, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | GNE fusion protein Ag18702 相同性解析による交差性が予測される生物種 |
| Full Name | glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase |
| Calculated molecular weight | 722 aa, 79 kDa |
| Observed molecular weight | 79 kDa |
| GenBank accession number | BC121179 |
| Gene symbol | GNE |
| Gene ID (NCBI) | 10020 |
| RRID | AB_2882849 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The bifunctional enzyme UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase (GNE) is essential for early embryonic development and catalyzes the rate limiting step in sialic acid biosynthesis. It plays an important role in neuronal cell and brain development, and is strongly involved in cardiac tissue and skeletal muscle early survival and organization.. This protein has 5 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GNE antibody 67649-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Neurol Sci Clinical, genetic, and pathological characterization of GNE myopathy in China. |
