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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated GNMT Monoclonal antibody

GNMT Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Pig, Rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B5E3

Cat no : CL488-67294

Synonyms

glycine N methyltransferase, GNMT

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CoraLite® Plus 488-conjugated GNMT Monoclonal anti (CL488-67294)

    IF Staining of A431 using CL488-67294

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 GNMT antibody (CL488-67294, Clone: 1B5E3 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67294 targets GNMT in IF/ICC applications and shows reactivity with Human, Mouse, Pig, Rat samples.

Tested Reactivity Human, Mouse, Pig, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GNMT fusion protein Ag4598 相同性解析による交差性が予測される生物種
Full Name glycine N-methyltransferase
Calculated molecular weight 295 aa, 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC032627
Gene symbol GNMT
Gene ID (NCBI) 27232
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glycine N-methyltransferase (GNMT, EC 2.1.1.20) was found originally as an enzyme regulating the ratio of SAM to S-adenosyl- homocysteine. GNMT is conservative among different animal species. Glycine-N methyltransferase (GNMT) is a potential tumor suppressor that is commonly inactivated in human hepatoma. GNMT is abundant in liver, but very low in HepG2 cells (PMID: 12566990).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 GNMT antibody CL488-67294Download protocol
Standard Protocols
Click here to view our Standard Protocols