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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GNPAT Monoclonal antibody

GNPAT Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2E5C10

Cat no : 66453-1-Ig

Synonyms

DAP AT, DAPAT, DHAP AT, DHAPAT, GNPAT

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using GNPAT Monoclonal antibody (66453-1-Ig)

    WB analysis of HeLa using 66453-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66453-1-Ig (GNPAT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using GNPAT Monoclonal antibody (66453-1-Ig)

    WB analysis of COLO 320 using 66453-1-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 66453-1-Ig (GNPAT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using GNPAT Monoclonal antibody (66453-1-Ig)

    WB analysis of HepG2 using 66453-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66453-1-Ig (GNPAT antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GNPAT Monoclonal antibody (66453-1-Ig)

    IHC staining of human liver cancer using 66453-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66453-1-Ig (GNPAT antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GNPAT Monoclonal antibody (66453-1-Ig)

    IHC staining of human liver cancer using 66453-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66453-1-Ig (GNPAT antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, COLO 320 cells, HepG2 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66453-1-Ig targets GNPAT in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen GNPAT fusion protein Ag6740 相同性解析による交差性が予測される生物種
Full Name glyceronephosphate O-acyltransferase
Calculated molecular weight 77 kDa
Observed molecular weight 65-69 kDa
GenBank accession numberBC000450
Gene symbol GNPAT
Gene ID (NCBI) 8443
RRIDAB_2881822
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GNPAT, also named as DAPAT and DHAPAT, belongs to the GPAT/DAPAT family. It is a key enzyme in the biosynthesis of ether phospholipids. GNPAT is localized exclusively within peroxisomes. Full GNPAT activity depends not only on the presence of AGPS, but also on the integrity of substrate channeling from GNPAT to AGPS. (PMID: 21990100) This antibody recognize the 65-69 kDa GNPAT protein.

Protocols

Product Specific Protocols
WB protocol for GNPAT antibody 66453-1-IgDownload protocol
IHC protocol for GNPAT antibody 66453-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols