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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using GNPAT Monoclonal antibody (66453-1-Ig)

    WB analysis of HeLa using 66453-1-Ig (same clone as 66453-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66453-1-Ig (GNPAT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66453-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using GNPAT Monoclonal antibody (66453-1-Ig)

    WB analysis of COLO 320 using 66453-1-Ig (same clone as 66453-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 66453-1-Ig (GNPAT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66453-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using GNPAT Monoclonal antibody (66453-1-Ig)

    WB analysis of HepG2 using 66453-1-Ig (same clone as 66453-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66453-1-Ig (GNPAT antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66453-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GNPAT Monoclonal antibody (66453-1-Ig)

    IHC staining of human liver cancer using 66453-1-Ig (same clone as 66453-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66453-1-Ig (GNPAT antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66453-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GNPAT Monoclonal antibody (66453-1-Ig)

    IHC staining of human liver cancer using 66453-1-Ig (same clone as 66453-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66453-1-Ig (GNPAT antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66453-1-PBS in a different storage buffer formulation.

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GNPAT Monoclonal antibody, PBS Only

GNPAT Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66453-1-PBS
Clone No.2E5C10

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

DAP AT, DAPAT, DHAP AT, DHAPAT, GNPAT

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66453-1-PBS targets GNPAT in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen GNPAT fusion protein Ag6740 相同性解析による交差性が予測される生物種
Full Name glyceronephosphate O-acyltransferase
Calculated molecular weight 77 kDa
Observed molecular weight 65-69 kDa
GenBank accession numberBC000450
Gene Symbol GNPAT
Gene ID (NCBI) 8443
RRIDAB_2881822
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO15228
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

GNPAT, also named as DAPAT and DHAPAT, belongs to the GPAT/DAPAT family. It is a key enzyme in the biosynthesis of ether phospholipids. GNPAT is localized exclusively within peroxisomes. Full GNPAT activity depends not only on the presence of AGPS, but also on the integrity of substrate channeling from GNPAT to AGPS. (PMID: 21990100) This antibody recognize the 65-69 kDa GNPAT protein.