Validation Data Gallery

Filter: AllWBIF/ICCFC (Intra)

WB analysis using 12640-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12640-1-AP (golgin 97 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of SMMC-7721 using 12640-1-AP
SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 12640-1-AP (golgin 97 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 12640-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12640-1-AP (golgin 97 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IF Staining of HeLa using 12640-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using golgin 97 antibody (12640-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of HeLa using 12640-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using golgin 97 antibody (12640-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 12640-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human golgin 97 (12640-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

golgin 97 Polyclonal antibody

golgin 97 Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 12640-1-AP

Publications(27)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

GOLGA1, Golgin-97, Golgin subfamily A member 1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (27)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, SMMC-7721 cells, HeLa cells, HepG2 cells
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunofluorescence (IF)/ICC	IF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 14 publications below
IHC	See 1 publications below
IF	See 16 publications below
IP	See 2 publications below

Product Information

12640-1-AP targets golgin 97 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	golgin 97 fusion protein Ag3335 相同性解析による交差性が予測される生物種
Full Name	golgi autoantigen, golgin subfamily a, 1
Calculated molecular weight	767 aa, 88 kDa
Observed molecular weight	97 kDa
GenBank accession number	BC032853
Gene Symbol	golgin 97
Gene ID (NCBI)	2800
RRID	AB_2115315
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q92805
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Golgin subfamily A member 1 is a protein that in humans is encoded by the GOLGA1 gene. GOLGA1 has been shown to interact with ARL1. GOLGA1 is associated with Sjogren's syndrome. The MW of this protein is 97 kDa, and Catalog#12640-1-AP specially recognises the 97 kDa protein. IF result shows strong Gogli staining.

Protocols

Product Specific Protocols
WB protocol for golgin 97 antibody 12640-1-AP	Download protocol
IF protocol for golgin 97 antibody 12640-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IP,IF	Cell The WDR11 complex is a receptor for acidic-cluster-containing cargo proteins Authors - Huaqing Deng View Article
	IF,WB	Nat Commun TBC1D23 mediates Golgi-specific LKB1 signaling Authors - Yingfeng Tu View Article
mouse	IF	Nat Commun Depletion of mitochondrial methionine adenosyltransferase α1 triggers mitochondrial dysfunction in alcohol-associated liver disease. Authors - Lucía Barbier-Torres View Article
human,mouse	IF	Redox Biol Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis Authors - Mengyue You View Article
human	WB	Dev Cell GRASP55 Senses Glucose Deprivation through O-GlcNAcylation to Promote Autophagosome-Lysosome Fusion. Authors - Xiaoyan Zhang View Article
human	WB	Cell Rep CDK5-PRMT1-WDR24 signaling cascade promotes mTORC1 signaling and tumor growth Authors - Shasha Yin View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 12640-1-AP

WB analysis of SMMC-7721 using 12640-1-AP

WB analysis using 12640-1-AP

IF Staining of HeLa using 12640-1-AP

IF Staining of HeLa using 12640-1-AP

FC experiment of HeLa using 12640-1-AP

golgin 97 Polyclonal antibody

golgin 97 Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The WDR11 complex is a receptor for acidic-cluster-containing cargo proteins

TBC1D23 mediates Golgi-specific LKB1 signaling

Depletion of mitochondrial methionine adenosyltransferase α1 triggers mitochondrial dysfunction in alcohol-associated liver disease.

Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis

GRASP55 Senses Glucose Deprivation through O-GlcNAcylation to Promote Autophagosome-Lysosome Fusion.

CDK5-PRMT1-WDR24 signaling cascade promotes mTORC1 signaling and tumor growth