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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated golgin 97 Polyclonal antibody

golgin 97 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-12640

Synonyms

GOLGA1, Golgin-97, Golgin subfamily A member 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated golgin 97 Polyclonal (CL488-12640)

    IF Staining of HeLa using CL488-12640

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 golgin 97 antibody (CL488-12640) at dilution of 1:400, CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated golgin 97 Polyclonal (CL488-12640)

    FC experiment of HeLa using CL488-12640

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human golgin 97 (CL488-12640) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-12640 targets golgin 97 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen golgin 97 fusion protein Ag3335 相同性解析による交差性が予測される生物種
Full Name golgi autoantigen, golgin subfamily a, 1
Calculated molecular weight 767 aa, 88 kDa
Observed molecular weight 97 kDa
GenBank accession numberBC032853
Gene symbol golgin 97
Gene ID (NCBI) 2800
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Golgin subfamily A member 1 is a protein that in humans is encoded by the GOLGA1 gene. GOLGA1 has been shown to interact with ARL1. GOLGA1 is associated with Sjogren's syndrome. The MW of this protein is 97 kDa. IF result shows strong Gogli staining.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 golgin 97 antibody CL488-12640Download protocol
FC protocol for CL Plus 488 golgin 97 antibody CL488-12640Download protocol
Standard Protocols
Click here to view our Standard Protocols