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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

golgin 97 Monoclonal antibody

golgin 97 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2A5E4

Cat no : 68648-1-Ig

Synonyms

GOLGA1, golgin 97, Golgin subfamily A member 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using golgin 97 Monoclonal antibody (68648-1-Ig)

    WB analysis using 68648-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68648-1-Ig (golgin 97 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using golgin 97 Monoclonal antibody (68648-1-Ig)

    IF Staining of MCF-7 using 68648-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using golgin 97 antibody (68648-1-Ig, Clone: 2A5E4 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using golgin 97 Monoclonal antibody (68648-1-Ig)

    IF Staining of HeLa using 68648-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using golgin 97 antibody (68648-1-Ig, Clone: 2A5E4 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).

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Tested Applications

Positive WB detected inA549 cells, LNCaP cells, MCF-7 cells, HeLa cells
Positive IF/ICC detected inMCF-7 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68648-1-Ig targets golgin 97 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen golgin 97 fusion protein Ag33790 相同性解析による交差性が予測される生物種
Full Name golgi autoantigen, golgin subfamily a, 1
Calculated molecular weight 767 aa, 88 kDa
Observed molecular weight 97 kDa
GenBank accession numberBC032853
Gene symbol golgin 97
Gene ID (NCBI) 2800
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Golgin subfamily A member 1 is a protein that in humans is encoded by the GOLGA1 gene. GOLGA1 has been shown to interact with ARL1. GOLGA1 is associated with Sjogren's syndrome. The MW of this protein is 97 kDa. IF result shows strong Gogli staining.

Protocols

Product Specific Protocols
WB protocol for golgin 97 antibody 68648-1-IgDownload protocol
IF protocol for golgin 97 antibody 68648-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols