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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

golgin 97 Monoclonal antibody, PBS Only (Detector)

golgin 97 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A5E4

Cat no : 68648-1-PBS

Synonyms

GOLGA1, Golgin-97, Golgin subfamily A member 1, 2A5E4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using golgin 97 Monoclonal antibody (68648-1-Ig)

    WB analysis using 68648-1-Ig (same clone as 68648-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68648-1-Ig (golgin 97 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68648-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using golgin 97 Monoclonal antibody (68648-1-Ig)

    IF Staining of MCF-7 using 68648-1-Ig (same clone as 68648-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using golgin 97 antibody (68648-1-Ig, Clone: 2A5E4 ) at dilution of 1:0 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). This data was developed using the same antibody clone with 68648-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using golgin 97 Monoclonal antibody (68648-1-Ig)

    IF Staining of HeLa using 68648-1-Ig (same clone as 68648-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using golgin 97 antibody (68648-1-Ig, Clone: 2A5E4 ) at dilution of 1:0 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). This data was developed using the same antibody clone with 68648-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50871-1

    Cytometric bead array standard curve of MP50871-1

    Cytometric bead array standard curve of MP50871-1, GOLGA1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68648-2-PBS. Detection antibody: 68648-1-PBS. Standard:Ag33790. Range: 0.195-100 ng/mL.

  • Cytometric bead array standard curve of MP50871-3

    Cytometric bead array standard curve of MP50871-3

    Cytometric bead array standard curve of MP50871-3, GOLGA1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68648-3-PBS. Detection antibody: 68648-1-PBS. Standard:Ag33790. Range: 1.563-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68648-1-PBS targets golgin 97 as part of a matched antibody pair:

MP50871-1: 68648-2-PBS capture and 68648-1-PBS detection (validated in Cytometric bead array)

MP50871-3: 68648-3-PBS capture and 68648-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen golgin 97 fusion protein Ag33790 相同性解析による交差性が予測される生物種
Full Name golgi autoantigen, golgin subfamily a, 1
Calculated molecular weight 767 aa, 88 kDa
Observed molecular weight 97 kDa
GenBank accession numberBC032853
Gene symbol golgin 97
Gene ID (NCBI) 2800
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Golgin subfamily A member 1 is a protein that in humans is encoded by the GOLGA1 gene. GOLGA1 has been shown to interact with ARL1. GOLGA1 is associated with Sjogren's syndrome. The MW of this protein is 97 kDa. IF result shows strong Gogli staining.