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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

golgin 97 Recombinant antibody, PBS Only

golgin 97 Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230523A5

Cat no : 83009-4-PBS

Synonyms

GOLGA1, Golgin-97, Golgin subfamily A member 1, 230523A5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using golgin 97 Recombinant antibody (83009-4-RR)

    WB analysis using 83009-4-RR (same clone as 83009-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83009-4-RR (golgin 97 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83009-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using golgin 97 Recombinant antibody (83009-4-RR)

    IF Staining of HepG2 using 83009-4-RR (same clone as 83009-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using golgin 97 antibody (83009-4-RR, Clone: 230523A5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83009-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using golgin 97 Recombinant antibody (83009-4-RR)

    FC experiment of HepG2 using 83009-4-RR (same clone as 83009-4-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug golgin 97 Recombinant antibody (83009-4-RR, Clone:230523A5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83009-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83009-4-RR

    Affinity and Kinetic Characterization of 83009-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83009-4-RR against Human golgin 97 were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83009-4-PBS is golgin 97 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen golgin 97 fusion protein Ag3335 相同性解析による交差性が予測される生物種
Full Name golgi autoantigen, golgin subfamily a, 1
Calculated molecular weight 767 aa, 88 kDa
Observed molecular weight 97 kDa
GenBank accession numberBC032853
Gene symbol golgin 97
Gene ID (NCBI) 2800
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Golgin subfamily A member 1 is a protein that in humans is encoded by the GOLGA1 gene. GOLGA1 has been shown to interact with ARL1. GOLGA1 is associated with Sjogren's syndrome. IF result shows strong Gogli staining.