golgin 97 Recombinant antibody

golgin 97 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230523A5

Cat no : 83009-4-RR

Synonyms

GOLGA1, Golgin-97, Golgin subfamily A member 1, 230523A5


☆大好評トライアルサイズ ¥20,000~! >>> 一次抗体特別価格キャンペーン開催中!


Tested Applications

Positive WB detected inHeLa cells, A549 cells, LNCaP cells, MCF-7 cells, SMMC-7721 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83009-4-RR is golgin 97 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen golgin 97 fusion protein Ag3335 相同性解析による交差性が予測される生物種
Full Name golgi autoantigen, golgin subfamily a, 1
Calculated molecular weight 767 aa, 88 kDa
Observed molecular weight 97 kDa
GenBank accession numberBC032853
Gene symbol golgin 97
Gene ID (NCBI) 2800
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Golgin subfamily A member 1 is a protein that in humans is encoded by the GOLGA1 gene. GOLGA1 has been shown to interact with ARL1. GOLGA1 is associated with Sjogren's syndrome. IF result shows strong Gogli staining.

Protocols

Product Specific Protocols
WB protocol for golgin 97 antibody 83009-4-RRDownload protocol
IHC protocol for golgin 97 antibody 83009-4-RRDownload protocol
IF protocol for golgin 97 antibody 83009-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols