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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GOT2 Monoclonal antibody (67738-1-Ig)

    WB analysis using 67738-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67738-1-Ig (GOT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using GOT2 Monoclonal antibody (67738-1-Ig)

    WB analysis using 67738-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67738-1-Ig (GOT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using GOT2 Monoclonal antibody (67738-1-Ig)

    IHC staining of mouse heart using 67738-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67738-1-Ig (GOT2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat heart tissue using GOT2 Monoclonal antibody (67738-1-Ig)

    IHC staining of rat heart using 67738-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67738-1-Ig (GOT2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using GOT2 Monoclonal antibody (67738-1-Ig)

    IHC staining of rat brain using 67738-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67738-1-Ig (GOT2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat heart tissue using GOT2 Monoclonal antibody (67738-1-Ig)

    IHC staining of rat heart using 67738-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67738-1-Ig (GOT2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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GOT2 Monoclonal antibody

GOT2 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67738-1-Ig
Clone No.1B6C6

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, chicken

Applications

WB, IHC, ELISA

Synonyms

FABP 1, FABPpm, Fatty acid binding protein, FLJ40994, GOT2, KAT4, KATIV, mAspAT, mitAAT, Transaminase A

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inpig brain tissue, A431 cells, rat brain, mouse brain, chicken brain, HEK-293 cells, HepG2 cells
Positive IHC detected inmouse heart tissue, rat brain tissue, rat heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67738-1-Ig targets GOT2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig, chicken samples.

Tested Reactivity Human, mouse, rat, pig, chicken
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GOT2 fusion protein Ag6600 相同性解析による交差性が予測される生物種
Full Name glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2)
Calculated molecular weight 47 kDa
Observed molecular weight39-40 kDa
GenBank accession numberBC000525
Gene Symbol GOT2
Gene ID (NCBI) 2806
RRIDAB_2918507
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP00505
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

GOT2 encodes the mitochondrial glutamate oxaloacetate transaminase and plays an essential role in the intracellular NAD(H) redox balance. Upregulation of GOT2 has been reported in various cancers, including breast cancer and pancreatic cancer. GOT2 mutation leads to autosomal recessive neurometabolic disorder.

Protocols

Product Specific Protocols
WB protocol for GOT2 antibody 67738-1-IgDownload protocol
IHC protocol for GOT2 antibody 67738-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols