Validation Data Gallery
Tested Applications
Positive WB detected in | pig brain tissue, A431 cells, rat brain, mouse brain, chicken brain, HEK-293 cells, HepG2 cells |
Positive IHC detected in | mouse heart tissue, rat brain tissue, rat heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:4000-1:16000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
67738-1-Ig targets GOT2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig, chicken samples.
Tested Reactivity | Human, mouse, rat, pig, chicken |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | GOT2 fusion protein Ag6600 相同性解析による交差性が予測される生物種 |
Full Name | glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) |
Calculated molecular weight | 47 kDa |
Observed molecular weight | 39-40 kDa |
GenBank accession number | BC000525 |
Gene Symbol | GOT2 |
Gene ID (NCBI) | 2806 |
RRID | AB_2918507 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P00505 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
GOT2 encodes the mitochondrial glutamate oxaloacetate transaminase and plays an essential role in the intracellular NAD(H) redox balance. Upregulation of GOT2 has been reported in various cancers, including breast cancer and pancreatic cancer. GOT2 mutation leads to autosomal recessive neurometabolic disorder.
Protocols
Product Specific Protocols | |
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WB protocol for GOT2 antibody 67738-1-Ig | Download protocol |
IHC protocol for GOT2 antibody 67738-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |