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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GOT2 Monoclonal antibody, PBS Only

GOT2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, chicken

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B6C6

Cat no : 67738-1-PBS

Synonyms

FABP 1, FABPpm, Fatty acid binding protein, FLJ40994, GOT2, KAT4, KATIV, mAspAT, mitAAT, Transaminase A

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using GOT2 Monoclonal antibody (67738-1-Ig)

    WB analysis using 67738-1-Ig (same clone as 67738-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67738-1-Ig (GOT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67738-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GOT2 Monoclonal antibody (67738-1-Ig)

    WB analysis using 67738-1-Ig (same clone as 67738-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67738-1-Ig (GOT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67738-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using GOT2 Monoclonal antibody (67738-1-Ig)

    IHC staining of mouse heart using 67738-1-Ig (same clone as 67738-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67738-1-Ig (GOT2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67738-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat heart tissue using GOT2 Monoclonal antibody (67738-1-Ig)

    IHC staining of rat heart using 67738-1-Ig (same clone as 67738-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67738-1-Ig (GOT2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67738-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using GOT2 Monoclonal antibody (67738-1-Ig)

    IHC staining of rat brain using 67738-1-Ig (same clone as 67738-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67738-1-Ig (GOT2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67738-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat heart tissue using GOT2 Monoclonal antibody (67738-1-Ig)

    IHC staining of rat heart using 67738-1-Ig (same clone as 67738-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 67738-1-Ig (GOT2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67738-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67738-1-PBS targets GOT2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig, chicken samples.

Tested Reactivity Human, mouse, rat, pig, chicken
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GOT2 fusion protein Ag6600 相同性解析による交差性が予測される生物種
Full Name glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2)
Calculated molecular weight 47 kDa
Observed molecular weight39-40 kDa
GenBank accession numberBC000525
Gene symbol GOT2
Gene ID (NCBI) 2806
RRIDAB_2918507
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GOT2 encodes the mitochondrial glutamate oxaloacetate transaminase and plays an essential role in the intracellular NAD(H) redox balance. Upregulation of GOT2 has been reported in various cancers, including breast cancer and pancreatic cancer. GOT2 mutation leads to autosomal recessive neurometabolic disorder.