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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GPD2 Monoclonal antibody (68174-1-Ig)

    WB analysis using 68174-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68174-1-Ig (GPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using GPD2 Monoclonal antibody (68174-1-Ig)

    WB analysis of HSC-T6 using 68174-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68174-1-Ig (GPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using GPD2 Monoclonal antibody (68174-1-Ig)

    WB analysis using 68174-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68174-1-Ig (GPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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GPD2 Monoclonal antibody

GPD2 Monoclonal Antibody for WB, ELISA
Cat No. 68174-1-Ig
Clone No.1C12E2

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse

Applications

WB, ELISA

Synonyms

GDH2, GPD M, GPD2, GPDH M, GPDM, mGPDH, mtGPD

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, HSC-T6 cells, NIH/3T3 cells, HeLa cells, 4T1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68174-1-Ig targets GPD2 in WB, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Reactivity Human, Rat, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GPD2 fusion protein Ag11212 相同性解析による交差性が予測される生物種
Full Name glycerol-3-phosphate dehydrogenase 2 (mitochondrial)
Calculated molecular weight 41 kDa, 81 kDa
GenBank accession numberBC019874
Gene Symbol GPD2
Gene ID (NCBI) 2820
RRIDAB_3085046
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP43304
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GPD2(Glycerol-3-phosphate dehydrogenase, mitochondrial) belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.It is phosphorylated only in capacitated hamster spermatozoa and is noncanonically localized in the acrosome and principal piece in human, mouse, rat, and hamster spermatozoa, though in somatic cells it is localized in the mitochondria. This noncanonical localization may imply a role of GPD2 in acrosome reaction and hyperactivation(PMID:19333995 ).

Protocols

Product Specific Protocols
WB protocol for GPD2 antibody 68174-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols