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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GPD2 Monoclonal antibody, PBS Only (Detector)

GPD2 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C12E2

Cat no : 68174-1-PBS

Synonyms

GPD M, GPD 2, Glycerol-3-phosphate dehydrogenase, mitochondrial, GDH2, EC:1.1.5.3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using GPD2 Monoclonal antibody (68174-1-Ig)

    WB analysis using 68174-1-Ig (same clone as 68174-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68174-1-Ig (GPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68174-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using GPD2 Monoclonal antibody (68174-1-Ig)

    WB analysis of HSC-T6 using 68174-1-Ig (same clone as 68174-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68174-1-Ig (GPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68174-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GPD2 Monoclonal antibody (68174-1-Ig)

    WB analysis using 68174-1-Ig (same clone as 68174-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68174-1-Ig (GPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68174-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50432-1

    Cytometric bead array standard curve of MP50432-1

    Cytometric bead array standard curve of MP50432-1, GPD2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68174-2-PBS. Detection antibody: 68174-1-PBS. Standard:Ag11212. Range: 0.391-200 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68174-1-PBS targets GPD2 as part of a matched antibody pair:

MP50432-1: 68174-2-PBS capture and 68174-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GPD2 fusion protein Ag11212 相同性解析による交差性が予測される生物種
Full Name glycerol-3-phosphate dehydrogenase 2 (mitochondrial)
Calculated molecular weight 41 kDa, 81 kDa
GenBank accession numberBC019874
Gene symbol GPD2
Gene ID (NCBI) 2820
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GPD2(Glycerol-3-phosphate dehydrogenase, mitochondrial) belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.It is phosphorylated only in capacitated hamster spermatozoa and is noncanonically localized in the acrosome and principal piece in human, mouse, rat, and hamster spermatozoa, though in somatic cells it is localized in the mitochondria. This noncanonical localization may imply a role of GPD2 in acrosome reaction and hyperactivation(PMID:19333995 ).