Validation Data Gallery
Tested Applications
| Positive WB detected in | SK-BR-3 cells, A549 cells, HT-1080 cells, RT-4 cells, MG-63 cells, FaDu cells, MDA-MB-468 cells, PC-12 cells, RAw 264.7 cells, 4T1 cells, HeLa cells, A375 cells, U-251 cells, THP-1 cells, U-937 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IHC | See 4 publications below |
| IF | See 4 publications below |
Product Information
66926-1-Ig targets GPNMB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | GPNMB fusion protein Ag26747 相同性解析による交差性が予測される生物種 |
| Full Name | glycoprotein (transmembrane) nmb |
| Calculated molecular weight | 64 kDa |
| Observed molecular weight | 64-70 kDa |
| GenBank accession number | BC011595 |
| Gene Symbol | GPNMB |
| Gene ID (NCBI) | 10457 |
| RRID | AB_2882253 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q14956 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GPNMB also known as HGFIN, osteoactivin, and DC-HIL, is a type I membrane glycoprotein involved in various biological processes, including inflammation, invasion and metastasis of malignant tumors, cell differentiation, and tissue regeneration. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB acts as an osteogenic factor that stimulates osteoblast differentiation in vivo and in vitro.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GPNMB antibody 66926-1-Ig | Download protocol |
| IHC protocol for GPNMB antibody 66926-1-Ig | Download protocol |
| IF protocol for GPNMB antibody 66926-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int Immunopharmacol Lactic acid-induced M2-like macrophages facilitate tumor cell migration and invasion via the GPNMB/CD44 axis in oral squamous cell carcinoma | ||
PLoS Negl Trop Dis Mining host candidate regulators of schistosomiasis-induced liver fibrosis in response to artesunate therapy through transcriptomics approach | ||
medRxiv Plasticity of Human Microglia and Brain Perivascular Macrophages in Aging and Alzheimer's Disease | ||
Cancers (Basel) TROP-2, Nectin-4, GPNMB, and B7-H3 Are Potentially Therapeutic Targets for Anaplastic Thyroid Carcinoma | ||
medRxiv The post-viral GPNMB + immune niche persists in long-term Covid, asthma, and COPD | ||
Neurochem Res Single-cell RNA Sequencing Identifies a Novel Subtype of Microglia with High Cd74 Expression that Facilitates White Matter Inflammation During Chronic Cerebral Hypoperfusion |