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GPNMB Monoclonal antibody

GPNMB Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66926-1-Ig
Clone No.2B10B8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

UNQ1725/PRO9925, Transmembrane glycoprotein NMB, Osteoactivin, NMB, Hematopoietic growth factor inducible neurokinin-1 type

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inSK-BR-3 cells, A549 cells, HT-1080 cells, RT-4 cells, MG-63 cells, FaDu cells, MDA-MB-468 cells, PC-12 cells, RAw 264.7 cells, 4T1 cells, HeLa cells, A375 cells, U-251 cells, THP-1 cells, U-937 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66926-1-Ig targets GPNMB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GPNMB fusion protein Ag26747 相同性解析による交差性が予測される生物種
Full Name glycoprotein (transmembrane) nmb
Calculated molecular weight 64 kDa
Observed molecular weight 64-70 kDa
GenBank accession numberBC011595
Gene Symbol GPNMB
Gene ID (NCBI) 10457
RRIDAB_2882253
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ14956
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GPNMB also known as HGFIN, osteoactivin, and DC-HIL, is a type I membrane glycoprotein involved in various biological processes, including inflammation, invasion and metastasis of malignant tumors, cell differentiation, and tissue regeneration. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB acts as an osteogenic factor that stimulates osteoblast differentiation in vivo and in vitro.

Protocols

Product Specific Protocols
WB protocol for GPNMB antibody 66926-1-IgDownload protocol
IHC protocol for GPNMB antibody 66926-1-IgDownload protocol
IF protocol for GPNMB antibody 66926-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF

Int Immunopharmacol

Lactic acid-induced M2-like macrophages facilitate tumor cell migration and invasion via the GPNMB/CD44 axis in oral squamous cell carcinoma

Authors - Ying Lin
WB

PLoS Negl Trop Dis

Mining host candidate regulators of schistosomiasis-induced liver fibrosis in response to artesunate therapy through transcriptomics approach

Authors - Yajie Yuan
human

medRxiv

Plasticity of Human Microglia and Brain Perivascular Macrophages in Aging and Alzheimer's Disease

Authors - Donghoon Lee
humanIHC

Cancers (Basel)

TROP-2, Nectin-4, GPNMB, and B7-H3 Are Potentially Therapeutic Targets for Anaplastic Thyroid Carcinoma

Authors - Soji Toda
humanIF

medRxiv

The post-viral GPNMB + immune niche persists in long-term Covid, asthma, and COPD

Authors - Kangyun Wu
ratIF

Neurochem Res

Single-cell RNA Sequencing Identifies a Novel Subtype of Microglia with High Cd74 Expression that Facilitates White Matter Inflammation During Chronic Cerebral Hypoperfusion

Authors - Wenchao Cheng
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