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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GPNMB Monoclonal antibody (66926-1-Ig)

    WB analysis using 66926-1-Ig (same clone as 66926-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66926-1-Ig (GPNMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66926-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GPNMB Monoclonal antibody (66926-1-Ig)

    WB analysis using 66926-1-Ig (same clone as 66926-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66926-1-Ig (GPNMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66926-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GPNMB Monoclonal antibody (66926-1-Ig)

    WB analysis using 66926-1-Ig (same clone as 66926-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66926-1-Ig (GPNMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66926-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using GPNMB Monoclonal antibody (66926-1-Ig)

    IHC staining of human liver using 66926-1-Ig (same clone as 66926-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66926-1-Ig (GPNMB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 66926-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using GPNMB Monoclonal antibody (66926-1-Ig)

    IHC staining of human liver using 66926-1-Ig (same clone as 66926-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66926-1-Ig (GPNMB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 66926-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GPNMB Monoclonal antibody (66926-1-Ig)

    IF Staining of HeLa using 66926-1-Ig (same clone as 66926-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GPNMB antibody (66926-1-Ig, Clone: 2B10B8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66926-1-PBS in a different storage buffer formulation.

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GPNMB Monoclonal antibody, PBS Only

GPNMB Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66926-1-PBS
Clone No.2B10B8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

UNQ1725/PRO9925, Transmembrane glycoprotein NMB, Osteoactivin, NMB, Hematopoietic growth factor inducible neurokinin-1 type

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66926-1-PBS targets GPNMB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GPNMB fusion protein Ag26747 相同性解析による交差性が予測される生物種
Full Name glycoprotein (transmembrane) nmb
Calculated molecular weight 64 kDa
Observed molecular weight 64-70 kDa
GenBank accession numberBC011595
Gene Symbol GPNMB
Gene ID (NCBI) 10457
RRIDAB_2882253
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ14956
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

GPNMB also known as HGFIN, osteoactivin, and DC-HIL, is a type I membrane glycoprotein involved in various biological processes, including inflammation, invasion and metastasis of malignant tumors, cell differentiation, and tissue regeneration. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB acts as an osteogenic factor that stimulates osteoblast differentiation in vivo and in vitro.