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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated GPR35 Polyclonal antibody

GPR35 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-55248

Synonyms

Kynurenic acid receptor, KYNA receptor, G-protein coupled receptor 35, G protein coupled receptor 35

Validation Data Gallery

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  • Flow cytometry (FC) experiment of A549 cells using CoraLite® Plus 647-conjugated GPR35 Polyclonal ant (CL647-55248)

    FC experiment of A549 using CL647-55248

    1X10^6 A549 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human GPR35 (CL647-55248) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-55248 targets GPR35 in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name G protein-coupled receptor 35
Calculated molecular weight 34 kDa
GenBank accession numberNM_005301
Gene symbol GPR35
Gene ID (NCBI) 2859
RRIDAB_2883650
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GPR35, also named as KYNA receptor, belongs to the G-protein coupled receptor 1 family. GPR35 acts as a receptor for kynurenic acid, an intermediate in the tryptophan metabolic pathway. The activity of GPR35 is mediated by G-proteins that elicit calcium mobilization and inositol phosphate production through G(qi/o) proteins (PMID:16754668). This antibody is specific to GPR35.

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 GPR35 antibody CL647-55248Download protocol
Standard Protocols
Click here to view our Standard Protocols