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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GPT/ALT1 Monoclonal antibody, PBS Only

GPT/ALT1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, rat, mouse

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5F6B5

Cat no : 67531-1-PBS

Synonyms

AAT1, Alanine aminotransferase 1, ALAT1, ALT1, GPT, GPT 1, GPT/ALT1, GPT1

Validation Data Gallery

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  • Western Blot (WB) analysis of rat liver tissue using GPT/ALT1 Monoclonal antibody (67531-1-Ig)

    WB analysis of rat liver using 67531-1-Ig (same clone as 67531-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 67531-1-Ig (GPT/ALT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67531-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GPT/ALT1 Monoclonal antibody (67531-1-Ig)

    WB analysis using 67531-1-Ig (same clone as 67531-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67531-1-Ig (GPT/ALT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67531-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using GPT/ALT1 Monoclonal antibody (67531-1-Ig)

    WB analysis of mouse liver using 67531-1-Ig (same clone as 67531-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 67531-1-Ig (GPT/ALT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67531-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig heart tissue using GPT/ALT1 Monoclonal antibody (67531-1-Ig)

    WB analysis of pig heart using 67531-1-Ig (same clone as 67531-1-PBS)

    pig heart tissue were subjected to SDS PAGE followed by western blot with 67531-1-Ig (GPT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67531-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig liver tissue using GPT/ALT1 Monoclonal antibody (67531-1-Ig)

    WB analysis of pig liver using 67531-1-Ig (same clone as 67531-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67531-1-Ig (GPT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67531-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GPT/ALT1 Monoclonal antibody (67531-1-Ig)

    IF Staining of HepG2 using 67531-1-Ig (same clone as 67531-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GPT antibody (67531-1-Ig, Clone: 5F6B5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67531-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67531-1-PBS targets GPT/ALT1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Pig, rat, mouse samples.

Tested Reactivity Human, Pig, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GPT/ALT1 fusion protein Ag10453 相同性解析による交差性が予測される生物種
Full Name glutamic-pyruvate transaminase (alanine aminotransferase)
Calculated molecular weight 496 aa, 55 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC018207
Gene symbol ALT1
Gene ID (NCBI) 2875
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GPT, also known as ALT1 (glutamate-pyruvate transaminase 1), catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.