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Validation Data Gallery

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  • Western Blot (WB) analysis of rat liver tissue using GPT/ALT1 Monoclonal antibody (67531-1-Ig)

    WB analysis of rat liver using 67531-1-Ig (same clone as 67531-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 67531-1-Ig (GPT/ALT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67531-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GPT/ALT1 Monoclonal antibody (67531-1-Ig)

    WB analysis using 67531-1-Ig (same clone as 67531-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67531-1-Ig (GPT/ALT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67531-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using GPT/ALT1 Monoclonal antibody (67531-1-Ig)

    WB analysis of mouse liver using 67531-1-Ig (same clone as 67531-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 67531-1-Ig (GPT/ALT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67531-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig heart tissue using GPT/ALT1 Monoclonal antibody (67531-1-Ig)

    WB analysis of pig heart using 67531-1-Ig (same clone as 67531-1-PBS)

    pig heart tissue were subjected to SDS PAGE followed by western blot with 67531-1-Ig (GPT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67531-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig liver tissue using GPT/ALT1 Monoclonal antibody (67531-1-Ig)

    WB analysis of pig liver using 67531-1-Ig (same clone as 67531-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67531-1-Ig (GPT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67531-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GPT/ALT1 Monoclonal antibody (67531-1-Ig)

    IF Staining of HepG2 using 67531-1-Ig (same clone as 67531-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GPT antibody (67531-1-Ig, Clone: 5F6B5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67531-1-PBS in a different storage buffer formulation.

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GPT/ALT1 Monoclonal antibody, PBS Only

GPT/ALT1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67531-1-PBS
Clone No.5F6B5

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, rat, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

AAT1, Alanine aminotransferase 1, ALAT1, ALT1, GPT, GPT 1, GPT/ALT1, GPT1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67531-1-PBS targets GPT/ALT1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Pig, rat, mouse samples.

Tested Reactivity Human, Pig, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GPT/ALT1 fusion protein Ag10453 相同性解析による交差性が予測される生物種
Full Name glutamic-pyruvate transaminase (alanine aminotransferase)
Calculated molecular weight 496 aa, 55 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC018207
Gene Symbol ALT1
Gene ID (NCBI) 2875
RRIDAB_2882750
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP24298
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

GPT, also known as ALT1 (glutamate-pyruvate transaminase 1), catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.