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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GPT/ALT1 Monoclonal antibody

GPT/ALT1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, rat, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

5F6B5

Cat no : 67531-1-Ig

Synonyms

AAT1, Alanine aminotransferase 1, ALAT1, ALT1, GPT, GPT 1, GPT/ALT1, GPT1

Validation Data Gallery

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  • Western Blot (WB) analysis of rat liver tissue using GPT/ALT1 Monoclonal antibody (67531-1-Ig)

    WB analysis of rat liver using 67531-1-Ig

    rat liver tissue were subjected to SDS PAGE followed by western blot with 67531-1-Ig (GPT/ALT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using GPT/ALT1 Monoclonal antibody (67531-1-Ig)

    WB analysis using 67531-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67531-1-Ig (GPT/ALT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using GPT/ALT1 Monoclonal antibody (67531-1-Ig)

    WB analysis of mouse liver using 67531-1-Ig

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 67531-1-Ig (GPT/ALT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig heart tissue using GPT/ALT1 Monoclonal antibody (67531-1-Ig)

    WB analysis of pig heart using 67531-1-Ig

    pig heart tissue were subjected to SDS PAGE followed by western blot with 67531-1-Ig (GPT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig liver tissue using GPT/ALT1 Monoclonal antibody (67531-1-Ig)

    WB analysis of pig liver using 67531-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67531-1-Ig (GPT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GPT/ALT1 Monoclonal antibody (67531-1-Ig)

    IF Staining of HepG2 using 67531-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GPT antibody (67531-1-Ig, Clone: 5F6B5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inhuman milk, pig heart tissue, pig liver tissue, rat liver tissue, mouse liver tissue, rabbit heart tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67531-1-Ig targets GPT/ALT1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Pig, rat, mouse samples.

Tested Reactivity Human, Pig, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GPT/ALT1 fusion protein Ag10453 相同性解析による交差性が予測される生物種
Full Name glutamic-pyruvate transaminase (alanine aminotransferase)
Calculated molecular weight 496 aa, 55 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC018207
Gene symbol ALT1
Gene ID (NCBI) 2875
RRIDAB_2882750
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GPT, also known as ALT1 (glutamate-pyruvate transaminase 1), catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.

Protocols

Product Specific Protocols
WB protocol for GPT/ALT1 antibody 67531-1-IgDownload protocol
IF protocol for GPT/ALT1 antibody 67531-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols