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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GPX4 (Human Specific) Recombinant antibody (82822-2-RR)

    WB analysis using 82822-2-RR (same clone as 82822-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82822-2-RR (GPX4 antibody) at dilution of 1:7500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82822-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using GPX4 (Human Specific) Recombinant antibody (82822-2-RR)

    WB analysis of HEK-293 using 82822-2-RR (same clone as 82822-2-PBS)

    WB result of GPX4 (Human Specific) antibody (82822-2-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPX4 (Human Specific) transfected HEK-293 cells. This data was developed using the same antibody clone with 82822-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82822-2-RR

    Affinity and Kinetic Characterization of 82822-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82822-2-RR against Human GPX4 (Human Specific) were performed. The affinity constant is below 1 pM.

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  • KD/KO Validated

GPX4 (Human Specific) Recombinant antibody, PBS Only

GPX4 (Human Specific) Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 82822-2-PBS
Clone No.6E9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

GPX4, GPx-4, GPx 4, EC:1.11.1.9, EC:1.11.1.12

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82822-2-PBS targets GPX4 (Human Specific) in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GPX4 (Human Specific) fusion protein Ag30650 相同性解析による交差性が予測される生物種
Full Name glutathione peroxidase 4 (phospholipid hydroperoxidase)
Observed molecular weight 20-23 kDa
GenBank accession numberBC021567
Gene Symbol GPX4
Gene ID (NCBI) 2879
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP36969
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701).82822-2-RR is human specific.