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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GPX4 (Human Specific) Recombinant antibody

GPX4 (Human Specific) Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

6E9

Cat no : 82822-2-RR

Synonyms

GPX4, GPx-4, GPx 4, EC:1.11.1.9, EC:1.11.1.12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GPX4 (Human Specific) Recombinant antibody (82822-2-RR)

    WB analysis using 82822-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82822-2-RR (GPX4 antibody) at dilution of 1:7500 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82822-2-RR

    Affinity and Kinetic Characterization of 82822-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82822-2-RR against Human GPX4 (Human Specific) were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:15000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

82822-2-RR targets GPX4 (Human Specific) in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GPX4 (Human Specific) fusion protein Ag30650 相同性解析による交差性が予測される生物種
Full Name glutathione peroxidase 4 (phospholipid hydroperoxidase)
Observed molecular weight 20-23 kDa
GenBank accession numberBC021567
Gene symbol GPX4
Gene ID (NCBI) 2879
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701).82822-2-RR is human specific.

Protocols

Product Specific Protocols
WB protocol for GPX4 (Human Specific) antibody 82822-2-RRDownload protocol
IF protocol for GPX4 (Human Specific) antibody 82822-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem Pharmacol

HDAC inhibitors activate lipid peroxidation and ferroptosis in gastric cancer

Authors - Robert Jenke
View Article