Validation Data Gallery
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Tested Applications
| Positive IF/ICC detected in | A549 cells |
| Positive FC (Intra) detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-24905 targets GRAMD1B in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GRAMD1B fusion protein Ag20670 相同性解析による交差性が予測される生物種 |
| Full Name | GRAM domain containing 1B |
| Calculated molecular weight | 738 aa, 85 kDa |
| Observed molecular weight | 85 kDa |
| GenBank accession number | BC107480 |
| Gene Symbol | GRAMD1B |
| Gene ID (NCBI) | 57476 |
| RRID | AB_3672780 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q3KR37 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GRAMD1B, also named as KIAA1201, contains a transmembrane region and two domains of known function; the GRAM domain and a VASt domain. It is predicted to localize in the nucleus, supported by several nuclear transport signals and nuclearly associated motifs. This highly conserved gene is found in a variety of vertebrates and invertebrates, however, it is not found in bacteria or fungi. There're some isoforms with MW 50 kDa and 81-87 kDa. With phosphor modification, it's about 90-110 kDa in WB detection.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 GRAMD1B antibody CL488-24905 | Download protocol |
| FC protocol for CL Plus 488 GRAMD1B antibody CL488-24905 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |