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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated GRB2 Monoclonal antibody

GRB2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1C5D11

Cat no : CL594-66880

Synonyms

Adapter protein GRB2, ASH, EGFRBP GRB2, GRB2, Grb3 3, MST084, MSTP084, Protein Ash, SH2/SH3 adapter GRB2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated GRB2 Monoclonal antibody (CL594-66880)

    IF Staining of HeLa using CL594-66880

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 GRB2 antibody (CL594-66880, Clone: 1C5D11 ) at dilution of 1:200, CL488-phalloidin (green).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®594-conjugated GRB2 Monoclonal antibody (CL594-66880)

    IF Staining of HeLa using CL594-66880

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 GRB2 antibody (CL594-66880, Clone: 1C5D11 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66880 targets GRB2 in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GRB2 fusion protein Ag0395 相同性解析による交差性が予測される生物種
Full Name growth factor receptor-bound protein 2
Calculated molecular weight 25 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC000631
Gene symbol GRB2
Gene ID (NCBI) 2885
RRIDAB_2920051
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GRB2 (growth factor receptor-bound protein 2) binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. N-SH3 domain of Grb2 was involved in the protein vesicular localization including amyloid-β protein precursor (AβPP). Involvement of GRB2 in Ras-signaling pathway has been reported, recent finding show that GRB2 may also play a complex role in T and B-cell antigen receptor signaling.

Protocols

Product Specific Protocols
IF protocol for CL594 GRB2 antibody CL594-66880Download protocol
Standard Protocols
Click here to view our Standard Protocols