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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated GREM2 Polyclonal antibody

GREM2 Polyclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF-P, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-13892

Synonyms

Gremlin2, Gremlin 2, GREM 2, DAND3, DAND 3

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using CoraLite® Plus 488-conjugated GREM2 Polyclonal ant (CL488-13892)

    IF Staining of mouse kidney using CL488-13892

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using CoraLite® Plus 488 GREM2 antibody (CL488-13892) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HCT 116 cells using CoraLite® Plus 488-conjugated GREM2 Polyclonal ant (CL488-13892)

    FC experiment of HCT 116 using CL488-13892

    1X10^6 HCT 116 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human GREM2 (CL488-13892) (red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF-P detected inmouse kidney tissue
Positive FC (Intra) detected inHCT 116 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-13892 targets GREM2 in IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GREM2 fusion protein Ag4854 相同性解析による交差性が予測される生物種
Full Name gremlin 2, cysteine knot superfamily, homolog (Xenopus laevis)
Calculated molecular weight 168 aa, 19 kDa
GenBank accession numberBC046632
Gene symbol GREM2
Gene ID (NCBI) 64388
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 GREM2 antibody CL488-13892Download protocol
FC protocol for CL Plus 488 GREM2 antibody CL488-13892Download protocol
Standard Protocols
Click here to view our Standard Protocols