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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GRIM19 Monoclonal antibody

GRIM19 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3A10C5

Cat no : 67414-1-Ig

Synonyms

B16.6, CDA016, CGI 39, CI B16.6, Complex I B16.6, GRIM 19, GRIM19, NDUFA13

Validation Data Gallery

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  • Western Blot (WB) analysis of LNCaP cells using GRIM19 Monoclonal antibody (67414-1-Ig)

    WB analysis of LNCaP using 67414-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67414-1-Ig (GRIM19 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using GRIM19 Monoclonal antibody (67414-1-Ig)

    WB analysis of HepG2 using 67414-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67414-1-Ig (GRIM19 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using GRIM19 Monoclonal antibody (67414-1-Ig)

    WB analysis of HEK-293 using 67414-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67414-1-Ig (GRIM19 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GRIM19 Monoclonal antibody (67414-1-Ig)

    WB analysis of HeLa using 67414-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67414-1-Ig (GRIM19 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GRIM19 Monoclonal antibody (67414-1-Ig)

    IHC staining of human liver cancer using 67414-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67414-1-Ig (GRIM19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GRIM19 Monoclonal antibody (67414-1-Ig)

    IHC staining of human liver cancer using 67414-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67414-1-Ig (GRIM19 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GRIM19 Monoclonal antibody (67414-1-Ig)

    IHC staining of human breast cancer using 67414-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67414-1-Ig (GRIM19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GRIM19 Monoclonal antibody (67414-1-Ig)

    IHC staining of human breast cancer using 67414-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67414-1-Ig (GRIM19 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inLNCaP cells, HEK-293 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67414-1-Ig targets GRIM19 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GRIM19 fusion protein Ag20855 相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 13
Calculated molecular weight16.5 kDa
Observed molecular weight 16-18 kDa
GenBank accession numberBC009189
Gene symbol GRIM19
Gene ID (NCBI) 51079
RRIDAB_2882654
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

GRIM19(Gene associated with retinoic and interferon-induced mortality 19 protein) is also named as NDUFA13 ,CI-B16.6 and belongs to the complex I NDUFA13 subunit family.t GRIM-19 may distribute in the mitochondria and/or nucleus depending on the cell type.Alternatively, its cellular localization may be regulated by post-translational modifications such as phosphorylation and acetylation. Lastly, GRIM-19 may exist in different isoforms that may be distributed in distinct cellular locations(PMID:12628925).

Protocols

Product Specific Protocols
WB protocol for GRIM19 antibody 67414-1-IgDownload protocol
IHC protocol for GRIM19 antibody 67414-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols