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Species Reactivity

Species Reactivity

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Conjugate

GRIM19 Monoclonal antibody, PBS Only (Capture/Detector)

GRIM19 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A10C5

Cat no : 67414-1-PBS

Synonyms

NDUFA13, CI B16.6, CGI-39, CGI 39, Cell death regulatory protein GRIM-19

Validation Data Gallery

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  • Western Blot (WB) analysis of LNCaP cells using GRIM19 Monoclonal antibody (67414-1-Ig)

    WB analysis of LNCaP using 67414-1-Ig (same clone as 67414-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67414-1-Ig (GRIM19 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67414-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using GRIM19 Monoclonal antibody (67414-1-Ig)

    WB analysis of HepG2 using 67414-1-Ig (same clone as 67414-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67414-1-Ig (GRIM19 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67414-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using GRIM19 Monoclonal antibody (67414-1-Ig)

    WB analysis of HEK-293 using 67414-1-Ig (same clone as 67414-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67414-1-Ig (GRIM19 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67414-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using GRIM19 Monoclonal antibody (67414-1-Ig)

    WB analysis of HeLa using 67414-1-Ig (same clone as 67414-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67414-1-Ig (GRIM19 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67414-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GRIM19 Monoclonal antibody (67414-1-Ig)

    IHC staining of human liver cancer using 67414-1-Ig (same clone as 67414-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67414-1-Ig (GRIM19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67414-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GRIM19 Monoclonal antibody (67414-1-Ig)

    IHC staining of human liver cancer using 67414-1-Ig (same clone as 67414-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67414-1-Ig (GRIM19 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67414-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GRIM19 Monoclonal antibody (67414-1-Ig)

    IHC staining of human breast cancer using 67414-1-Ig (same clone as 67414-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67414-1-Ig (GRIM19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67414-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GRIM19 Monoclonal antibody (67414-1-Ig)

    IHC staining of human breast cancer using 67414-1-Ig (same clone as 67414-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67414-1-Ig (GRIM19 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67414-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50735-1

    Cytometric bead array standard curve of MP50735-1

    Cytometric bead array standard curve of MP50735-1, GRIM19 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67414-2-PBS. Detection antibody: 67414-1-PBS. Standard:Ag20855. Range: 0.098-100 ng/mL.

  • Cytometric bead array standard curve of MP50735-3

    Cytometric bead array standard curve of MP50735-3

    Cytometric bead array standard curve of MP50735-3, GRIM19 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67414-1-PBS. Detection antibody: 67414-4-PBS. Standard:Ag20855. Range: 0.098-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67414-1-PBS targets GRIM19 as part of a matched antibody pair:

MP50735-1: 67414-2-PBS capture and 67414-1-PBS detection (validated in Cytometric bead array)

MP50735-3: 67414-1-PBS capture and 67414-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GRIM19 fusion protein Ag20855 相同性解析による交差性が予測される生物種
Full Name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 13
Calculated molecular weight16.5 kDa
Observed molecular weight 16-18 kDa
GenBank accession numberBC009189
Gene symbol GRIM19
Gene ID (NCBI) 51079
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

GRIM19(Gene associated with retinoic and interferon-induced mortality 19 protein) is also named as NDUFA13 ,CI-B16.6 and belongs to the complex I NDUFA13 subunit family.t GRIM-19 may distribute in the mitochondria and/or nucleus depending on the cell type.Alternatively, its cellular localization may be regulated by post-translational modifications such as phosphorylation and acetylation. Lastly, GRIM-19 may exist in different isoforms that may be distributed in distinct cellular locations(PMID:12628925).