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  • Featured Product
  • KD/KO Validated

GRP78,BIP Recombinant antibody, PBS Only

GRP78,BIP Recombinant Antibody for WB, IP, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IP, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5F17

Cat no : 80849-1-PBS

Synonyms

BIP, FLJ26106, GRP 78, GRP78, Heat shock 70 kDa protein 5, HSPA5, MIF2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GRP78,BIP Recombinant antibody (80849-1-RR)

    WB analysis using 80849-1-RR (same clone as 80849-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80849-1-RR (GRP78,BIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80849-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using GRP78,BIP Recombinant antibody (80849-1-RR)

    WB analysis of HepG2 using 80849-1-RR (same clone as 80849-1-PBS)

    WB result of GRP78,BIP antibody (80849-1-RR; 1:16000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GRP78,BIP transfected HepG2 cells. This data was developed using the same antibody clone with 80849-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using GRP78,BIP Recombinant antibody (80849-1-RR)

    IP experiment of HeLa using 80849-1-RR (same clone as 80849-1-PBS)

    IP result of anti-GRP78,BIP (IP:80849-1-RR, 4ug; Detection:80849-1-RR 1:3000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 80849-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using GRP78,BIP Recombinant antibody (80849-1-RR)

    IHC staining of human colon cancer using 80849-1-RR (same clone as 80849-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80849-1-RR (GRP78,BIP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80849-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using GRP78,BIP Recombinant antibody (80849-1-RR)

    IHC staining of human pancreas cancer using 80849-1-RR (same clone as 80849-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 80849-1-RR (GRP78,BIP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80849-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GRP78,BIP Recombinant antibody (80849-1-RR)

    IHC staining of human breast cancer using 80849-1-RR (same clone as 80849-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80849-1-RR (GRP78,BIP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80849-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80849-1-PBS targets GRP78,BIP in WB, IP, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GRP78,BIP fusion protein Ag2188 相同性解析による交差性が予測される生物種
Full Name heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
Calculated molecular weight 78 kDa
Observed molecular weight70,78 kDa
GenBank accession numberBC020235
Gene symbol HSPA5
Gene ID (NCBI) 3309
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.