GRP78,BIP Recombinant antibody, PBS Only

WB result of GRP78,BIP antibody (80849-1-RR; 1:16000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GRP78,BIP transfected HepG2 cells. This data was developed using the same antibody clone with 80849-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 80849-1-RR (GRP78,BIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80849-1-PBS in a different storage buffer formulation.

IP result of anti-GRP78,BIP (IP:80849-1-RR, 4ug; Detection:80849-1-RR 1:3000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 80849-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80849-1-RR (GRP78,BIP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80849-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 80849-1-RR (GRP78,BIP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80849-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80849-1-RR (GRP78,BIP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80849-1-PBS in a different storage buffer formulation.

Biolayer interferometry (BLl) kinetic assays of 80849-1-RR against Human GRP78/BIP were performed. The affinity constant is 4.08 nM.