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  • Featured Product
  • KD/KO Validated

GRP78,BIP Recombinant antibody

GRP78,BIP Recombinant Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

5F17

Cat no : 80849-1-RR

Synonyms

BIP, GRP78, GRP78/BIP, HSPA5, Endoplasmic reticulum chaperone BiP

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using GRP78,BIP Recombinant antibody (80849-1-RR)

    WB analysis of HepG2 using 80849-1-RR

    WB result of GRP78,BIP antibody (80849-1-RR; 1:16000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GRP78,BIP transfected HepG2 cells.

  • Western Blot (WB) analysis of various lysates using GRP78,BIP Recombinant antibody (80849-1-RR)

    WB analysis using 80849-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80849-1-RR (GRP78,BIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using GRP78,BIP Recombinant antibody (80849-1-RR)

    IP experiment of HeLa using 80849-1-RR

    IP result of anti-GRP78,BIP (IP:80849-1-RR, 4ug; Detection:80849-1-RR 1:3000) with HeLa cells lysate 1280 ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using GRP78,BIP Recombinant antibody (80849-1-RR)

    IHC staining of human colon cancer using 80849-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80849-1-RR (GRP78,BIP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using GRP78,BIP Recombinant antibody (80849-1-RR)

    IHC staining of human pancreas cancer using 80849-1-RR

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 80849-1-RR (GRP78,BIP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GRP78,BIP Recombinant antibody (80849-1-RR)

    IHC staining of human breast cancer using 80849-1-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80849-1-RR (GRP78,BIP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 80849-1-RR

    Affinity and Kinetic Characterization of 80849-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80849-1-RR against Human GRP78/BIP were performed. The affinity constant is 4.08 nM.

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Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, RAW264.7 cells, HSC-T6 cells, pig liver tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, human pancreas cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

80849-1-RR targets GRP78,BIP in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivitypig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GRP78,BIP fusion protein Ag2188 相同性解析による交差性が予測される生物種
Full Name heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
Calculated molecular weight 78 kDa
Observed molecular weight70,78 kDa
GenBank accession numberBC020235
Gene symbol GRP78/BIP
Gene ID (NCBI) 3309
RRIDAB_2918916
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GRP78,BIP antibody 80849-1-RRDownload protocol
IHC protocol for GRP78,BIP antibody 80849-1-RRDownload protocol
IP protocol for GRP78,BIP antibody 80849-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigIF

J Agric Food Chem

Pterostilbene Prevents Tunicamycin-Induced Intestinal Barrier Damage by Targeting Endoplasmic Reticulum Stress, Oxidative Stress, Autophagy, and Gut Microbiota

Authors - Yanan Chen
View Article