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GRP78,BIP Recombinant antibody

GRP78,BIP Uni-rAbTM Recombinant Antibody for WB, IHC, IP, ELISA
Cat No. 80849-1-RR
Clone No.5F17

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF, IP, ELISA

BIP, GRP78, GRP78/BIP, HSPA5, Endoplasmic reticulum chaperone BiP

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, RAW264.7 cells, HSC-T6 cells, pig liver tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, human pancreas cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

80849-1-RR targets GRP78,BIP in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivitypig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GRP78,BIP fusion protein Ag2188 相同性解析による交差性が予測される生物種
Full Name heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
Calculated molecular weight 78 kDa
Observed molecular weight70,78 kDa
GenBank accession numberBC020235
Gene Symbol GRP78/BIP
Gene ID (NCBI) 3309
RRIDAB_2918916
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11021
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GRP78,BIP antibody 80849-1-RRDownload protocol
IHC protocol for GRP78,BIP antibody 80849-1-RRDownload protocol
IP protocol for GRP78,BIP antibody 80849-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigIF

J Agric Food Chem

Pterostilbene Prevents Tunicamycin-Induced Intestinal Barrier Damage by Targeting Endoplasmic Reticulum Stress, Oxidative Stress, Autophagy, and Gut Microbiota

Authors - Yanan Chen
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