- Featured Product
- KD/KO Validated
CoraLite® Plus 488-conjugated GRP78/BIP Polyclonal antibody
GRP78/BIP Polyclonal Antibody for IF
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-11587
Synonyms
Validation Data Gallery
Tested Applications
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-11587 targets GRP78/BIP in IF applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | GRP78/BIP fusion protein Ag2188 相同性解析による交差性が予測される生物種 |
| Full Name | heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) |
| Calculated molecular weight | 78 kDa |
| Observed molecular weight | 70-78 kDa |
| GenBank accession number | BC020235 |
| Gene symbol | HSPA5 |
| Gene ID (NCBI) | 3309 |
| RRID | AB_2919034 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
Background Information
GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).It is a constitutively expressed resident protein of the ER in all eukaryotic cells. Recently it has been reported that GRP78 is associated with apoptosis or inhibition of cancer cell growth. 11587-1-AP antibody recognizes one or two bands with MW 70-78 kDa for the signal peptide of GRP78 protein can be finally cleaved.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 GRP78/BIP antibody CL488-11587 | Download protocol |
| FC protocol for CL Plus 488 GRP78/BIP antibody CL488-11587 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
