CoraLite®594-conjugated GRP94 Monoclonal antibody
GRP94 Monoclonal Antibody for WB, IF/ICC, FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
WB, IF/ICC, FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
4G7C7
Cat no : CL594-60012
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cells, HepG2 cells |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HeLa cells |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-60012 targets GRP94 in WB, IF/ICC, FC (Intra) applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | GRP94 fusion protein Ag1439 相同性解析による交差性が予測される生物種 |
| Full Name | heat shock protein 90kDa beta (Grp94), member 1 |
| Calculated molecular weight | 96 kDa |
| Observed molecular weight | 95 kDa |
| GenBank accession number | BC009195 |
| Gene symbol | GRP94 |
| Gene ID (NCBI) | 7184 |
| RRID | AB_2919888 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
HSP90 proteins are highly conserved molecular chaperones, which normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 (GP96 or GRP94) is an endoplasmic reticulum paralogue of the cytosolic HSP90. As a major ER chaperone to mediate the UPR and a master chaperone for Toll-like receptors (TLRs), HSP90b1 chaperones peptides to MHC class I molecules of dendritic cells and other antigen-presenting cells, as well as facilitating the assembly of immunoglobulin. The protein is also involved in many other bio-processes. This antibody was generated against the N-terminal region of full-length HSP90b1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CL594 GRP94 antibody CL594-60012 | Download protocol |
| IF protocol for CL594 GRP94 antibody CL594-60012 | Download protocol |
| FC protocol for CL594 GRP94 antibody CL594-60012 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
