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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GSDMB Monoclonal antibody

GSDMB Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2F12C4

Cat no : 67527-1-Ig

Synonyms

gasdermin B, Gasdermin like protein, GSDMB, GSDML, PP4052, PRO2521

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using GSDMB Monoclonal antibody (67527-1-Ig)

    WB analysis of MCF-7 using 67527-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67527-1-Ig (GSDMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using GSDMB Monoclonal antibody (67527-1-Ig)

    WB analysis of PC-3 using 67527-1-Ig

    PC-3 cells were subjected to SDS PAGE followed by western blot with 67527-1-Ig (GSDMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using GSDMB Monoclonal antibody (67527-1-Ig)

    WB analysis of COLO 320 using 67527-1-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 67527-1-Ig (GSDMB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GSDMB Monoclonal antibody (67527-1-Ig)

    IF Staining of HepG2 using 67527-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GSDMB antibody (67527-1-Ig, Clone: 2F12C4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inMCF-7 cells, COLO 320 cells, PC-3 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67527-1-Ig targets GSDMB in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GSDMB fusion protein Ag28332 相同性解析による交差性が予測される生物種
Full Name gasdermin B
Calculated molecular weight 411 aa, 47 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC025682
Gene symbol GSDMB
Gene ID (NCBI) 55876
RRIDAB_2882746
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GSDMB antibody 67527-1-IgDownload protocol
IF protocol for GSDMB antibody 67527-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Arch Biochem Biophys

GSDMB involvement in the pathogenesis of abdominal aortic aneurysm through regulation of macrophage non-canonical pyroptosis

Authors - Xiaoping Xie
View Article